Department of Biochemistry

Duke University Medical Center

 

 

 

Duke University       

Medical Center

Department of Biochemistry

 

 

 

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Dr. Pei Zhou

Associate Professor of Biochemistry



Contact Information

Tel. (919) 668-6409
Fax (919) 684-8885
Email peizhou@biochem.duke.edu

Lab Location
Rm 270, Sands Building

Mailing Address
Department of Biochemistry
Box 3711, DUMC
Durham, NC 27710


Education

Ph.D. (Harvard University, 1998)

 


News

 

Read more in The Chronicle

4/10/2013.  Congratulations to Yaohua (Jenny) Xue, a Duke junior working in the lab, who has won the prestigious 2013 Barry M. Goldwater Scholarship!   

Read comments in Faculty of 1000.

1/30/2013.  Congratulations to Jessica Wojtaszek and Chul-Jin Lee! Their article: Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ζ, and Pol κ., JBC, 2012; 287(40):33836-46, has been recommended as being of special significance in its field from the F1000 Faculty: http://f1000.com/prime/717972978.

12/20/2012.  Congratulations to Jessica Wojtaszek, who has been awarded the Jo Rae Wright Fellowship for Outstanding Women in Science for academic year 2013-2014.

Prof. Brian Coggins will present his development of the SCRUB technology for efficient collection of high resolution 4-D NOESY spectra at the 2013 Keystone Symposia. This work is described in JACS 2012; 134(45): 18619-30. 

World Health Day – 7 April 2011

Antimicrobial resistance: no action today, no cure tomorrow    (Read more)

The March, 2011  issue of The Economist published a special report on antibiotic resistance: The spread of superbugs. (Read more

Prof. Pei Zhou spoke at the Cambridge Healthtech Institute's (CHI) PEPTALK conference (Jan 10-14, 2011) at San Diego, CA on using Solubility-enhancement tags (SETs) for solution NMR studies of challenging proteins. 

Congratulations to Jon Werner-Allen, who has been invited for an oral presentation at the Keystone Symposium: Frontiers of NMR in Biology, Jan 8 - Jan 13, 2011, Big Sky Resort in Big Sky, Montana.

Congratulations to Su Wang for winning the Graduate Student Poster Award at the 2010 Duke Biochemistry Departmental Retreat at the Wrightsville Beach, NC (09/17/2010-09/19/2010).


Research Interests
Protein-protein interactions play a pivotal role in the regulation of various cellular processes. The formation of higher order protein complexes is frequently accompanied by extensive structural remodeling of the individual components, varying from domain re-orientation to induced folding of unstructured elements. Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for macromolecular structure determination in solution. It has the unique advantage of being capable of elucidating the dynamic behavior of proteins during the process of recognition. Recent advances in NMR techniques have enabled the study of significantly larger proteins and protein complexes. These innovations have also led to faster and more accurate structure determination. My research interests focus on the exploration of molecular recognition and conformational variability of protein complexes in crucial biomedical processes using state-of-the-art NMR techniques. (More)

 


Recent Publications

 

 

--------- 2014 ---------

63. Structural basis of lipid binding for the membrane-embedded tetraacyldisaccharide-1-phosphate 4'-kinase LpxK. Emptage RP, Tonthat NK, York JD, Schumacher MA, and Zhou P. J. Biol. Chem. (doi:10.1074/jbc.M114.589986)

62. A CRISPR-Cas system enhances envelope integrity mediating antibiotic resistance and inflammasome evasion. Sampson TR, Napier BA, Schroeder MR, Louwen R, Zhao J, Chin  C-Y, Ratner HK, Llewellyn AC, Jones CL, Laroui H, Merlin D, Zhou P, Endtz HP, and Weiss DS. PNAS 2014; doi:10.1073/pnas.1323025111.

61. Sparsely-sampled, high-resolution 4-D omit spectra for detection and assignment of intermolecular NOEs of protein complexes. Wang S and Zhou P.  J Biomol NMR 2014; 59: 51-6 (doi: 10.1007/s10858-014-9834-2).

60. Chasing acyl carrier protein through a catalytic cycle of lipid A production. Masoudi A, Raetz CRH, Zhou P, and Pemble CW. Nature. 2014 Jan 16;505(7483):422-6. doi: 10.1038/nature12679.

59. Structural Basis of the Promiscuous Inhibitor Susceptibility of E. coli LpxC. Lee C-J, Liang X, Gopalaswamy R, Najeeb J, Ark, ED, Toone EJ, and Zhou P. ACS Chem. Biol. 2014 Jan 17;9(1):237-46. doi: 10.1021/cb400067g. (link)

--------- 2013 ---------

Recommended by
Faculty of 1000

58. Crystal Structure of MraY, an Essential Membrane Enzyme for Bacterial Cell Wall Synthesis. Chung BC, Zhao J, Gillespie RA, Kwon DY, Guan Z, Hong J, Zhou P, Lee SY. Science 2013; 341(6149): 1012-6. (PMC link)

57. Synthesis, structure and antibiotic activity of aryl-substituted LpxC inhibitors. Liang X, Lee C-J, Zhao J, Toone EJ, and Zhou P. J. Med. Chem., 2013, ASAP.

56. The UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis utilizes A Mn2+ cluster for catalysis. Young HE, Donohue MP, Smirnova TI, Smirnov AI, and Zhou P. J. Biol. Chem. published July 29, 2013 as doi:10.1074/jbc.M113.497636

55. Storage of hydrogen spin polarization in long-lived (13)c2 singlet order and implications for hyperpolarized magnetic resonance imaging. Feng Y, Theis T, Liang X, Wang Q, Zhou P, Warren WS. JACS. 2013; 135(26): 9632-5.  (link)

54. Mechanistic characterization of the tetraacyldisaccharide-1-phosphate 4´-kinase LpxK involved in lipid A biosynthesis. Emptage RP, Pemble CW, York JD, Raetz CR, Zhou P. Biochemistry. 2013; 52, 2280-90. (PDF)  

53. Specific interaction of the transcription elongation regulator TCERG1 with RNA polymerase II requires simultaneous phosphorylaHASH: a Program to Accurately Predict Protein Ha Shifts from Neighboring Backbone tion at Ser2, Ser5 and Ser7 within the carboxyl-terminal domain repeat. Liu J, Fan S, Lee CJ, Greenleaf AL, Zhou P. J Biol Chem. 2013; 288, 10890-901. (link)

52. Mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis. Zeng D, Zhao J, Chung HS, Guan Z, Raetz CR, Zhou P. J Biol Chem. 2013; 288: 5475-86. (link)

51. HASH: a Program to Accurately Predict Protein Ha Shifts from Neighboring Backbone Shifts. Zeng J, Zhou P, and Donald BR. J Biomol NMR 2013; 55: 105-18.  (PDF).

--------- 2012 ---------

50. Rapid protein global fold determination using ultrasparse sampling, high-dynamic range artifact suppression, and time-shared NOESY. Coggins BE, Werner-Allen JW, Yan A, and Zhou P. J Am Chem Soc. 2012; 134(45): 18619-30.  (PDF)

Recommended by Faculty of 1000

49. Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric Pol zeta and Pol kappa.  Wojtaszek J, Lee CJ, D'Souza S, Minesinger B, Kim H, D'Andrea AD, Walker GC, and Zhou P. J Biol Chem. 2012 Aug 2 (link)

48. Multifaceted recognition of vertebrate Rev1 by translesion polymerases zeta and kappa. Wojtaszek J, Liu J, D'Souza S, Wang S, Xue Y, Walker GC, Zhou P. J Biol Chem. 2012 July 27; 287 (31): 26400-8.  (link)

47. Efficient acquisition of high-resolution 4-D diagonal-suppressed methyl-methyl NOESY for large proteins. Wen J, Zhou P, Wu J. J Magn Reson. 2012 May;218:128-32.  PMID: 22464875. (PDF)

--------- 2011 ---------

46. Protein loop closure using orientational restraints from NMR data.Tripathy C, Zeng J, Zhou P, Donald BR. Proteins. 2011 Sep 26. doi: 10.1002/prot.23207. (PDF)

45. A geometric arrangement algorithm for structure determination of symmetric protein homo-oligomers from NOEs and RDCs. Martin JW, Yan AK, Bailey-Kellogg C, Zhou P, Donald BR. J Comput Biol. 2011 Nov;18(11):1507-23. Epub 2011 Oct 28.

44. A Bayesian approach for determining protein side-chain rotamer conformations using unassigned NOE data. Zeng J, Roberts KE, Zhou P, Donald BR. J Comput Biol. 2011 Nov;18(11):1661-79.

43. 1H, 13C and 15N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72. Werner-Allen JW, Zhou P. Biomol NMR Assign. 2011 Jul 6. (PDF)

42. Protein Side-Chain Resonance Assignment and NOE Assignment Using RDC-Defined Backbones without TOCSY Data. Zeng J, Zhou P and Donald BR. J Biomol NMR. 2011 Jun 25. (PDF)

41. Lipooligosaccharide is required for the generation of infectious elementary bodies in Chlamydia trachomatis. Nguyen BD, Cunningham D, Liang X, Chen X, Toone EJ, Raetz CR, Zhou P, Valdivia RH. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10284-9. (PDF)

40. A graphical method for analyzing distance restraints using residual dipolar couplings for structure determination of symmetric protein homo-oligomers. Martin JW, Yam AK, Bailey-Kellogg C, Zhou P and Donald BR. Protein Sci 2011; 20: 970-85. (PDF)

39. Sparsely-sampled high-resolution 4-D experiments for efficient backbone resonance assignment of disordered proteins. Wen J, Wu J, and Zhou P. J Magn Reson 209, 94-100 (2011). (PDF)
38. Cis proline-mediated pSer5-dephosphorylation by the RNA polymerase II CTD phosphatase Ssu72. Werner-Allen JW, Lee CJ, Liu P, Nicely NI, Wang S, Greenleaf AL, Zhou P. Journal of Biological Chemistry 286, 5717-5826 (2011). (PDB: 3P3Y) (PDF)
37. Syntheses, structures and antibiotic acitivities of LpxC inhibitors based on the diacetylene scaffold. Liang X, Lee CJ, Chen X, Chung HS, Zeng D, Raetz CRH, Li Y, Zhou P, Toone EJ. Bioorganic & Medicinal Chemistry 19: 852-60 (2011). (PDB: 3PS1, 3PS2, 3PS3) (PDF)
36. Species-specific and inhibitor-dependent conformations of LpxC-Implications for antibiotic design. Lee CJ, Liang X, Chen X, Zeng D, Joo SH, Chung HS, Barb AW, Swanson SM, Nicholas RA, Li Y, Toone EJ, Raetz CRH, Zhou P. Chemistry and Biology 18: 38-47 (2011). (PDB: 3P3C, 3P3E, 3P3G) (PDF)

 

(+) --------- 2009-2010 ----------

(+) --------- 2006-2008 ----------

(+) --------- 2003-2005 ----------

(+) Ph.D. and Postdoctoral Research

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